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The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of the gene is observed in several tumors, which ends up in chromosome instability, and therefore might add to tumorigenesis. This protein was observed to associate with, and be associated with, the phosphorylation of NPAT protein (nuclear protein mapped towards the ATM locus), which participates in cell-cycle regulated histone gene expression and performs a important position in marketing mobile-cycle development within the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode unique isoforms, have been explained. Two added splice variants were claimed but thorough nucleotide sequence details isn't but readily available.
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Blocking peptides are peptides that bind specially for the goal antibody and block antibody binding. These peptide commonly is made up of the epitope acknowledged with the antibody.
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This information outlines even further feasible optimization of cellular and immunoassay parameters to be certain the very best outcomes are...
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This guideline outlines further feasible optimization of mobile and immunoassay parameters to be sure the very best final results are...
Antibodies sure to the blocking peptide no more bind to the epitope about the target protein. By comparing the staining from your blocked antibody compared to the antibody by yourself, one can see which staining is certain.
Focus on Information and facts Cyclin E belongs to your highly conserved cyclin family, whose customers are characterised by a extraordinary periodicity in protein abundance from the mobile cycle. Cyclins function as regulators of CDK kinases. Distinct cyclins show distinct expression and degradation styles which lead to the temporal coordination of each mitotic party. Cyclin E varieties a fancy with and capabilities for a regulatory subunit of CDK2, whose activity is needed for mobile cycle G1/S transition. This protein accumulates within the G1-S stage boundary and it is degraded as cells progress as a result of S stage.
The measurement of protein phosphorylation is a useful tool for measuring the modulation of receptor activation by both equally antibodies...
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